GENOMIX COVID-19 REALTIME RT-PCR KIT

Genomix COVID-19 Assay is an in vitro diagnostic (IVD) real-time reverse transcriptase polymerase
chain reaction (RT-PCR) test intended for the qualitative detection of SARS-CoV-2 viral nucleic acids in human
nasopharyngeal swab, oropharyngeal swab, anterior nasal swab, mid-turbinate and sputum specimens from
individuals who are suspected of COVID-19.
The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction,
the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye
only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by
the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an
increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR
product. Monitoring the fluorescence intensities in real time allows the detection of the accumulating product
without having to re-open the reaction tube after the amplification. Real time reverse-transcription
polymerase chain reaction (real-time RT-PCR) is used when the starting material is RNA. In this method, RNA is
first transcribed into the complementary DNA (cDNA) byreverse transcriptase from total RNA. The cDNA is
then used as a template for the real time PCR.