HBV rapid diagnostic kit is visual, rapid, sensitive and accurate one step immunoassay for the qualitative detection of Hepatitis B surface antigen (HBsAg) in Human serum or plasma. The assay is intended to be used as an aid in the recognition and diagnosis of acute infections and chronic infections carriers of the Hepatitis B Virus (HBV).
Hepatitis B rapid kit is a one step immunoassay based on the antigen capture, or "sandwich" principle. The method uses monoclonal antibodies conjugated to colloidal gold and polyclonal antibodies immobilized on a nitrocellulose strip in a thin line. The test sample is introduced to and flows laterally through an absorbent pad where it mixes with the signal reagent. If the sample contains HBsAg, the colloidal gold-antibody conjugate binds to the antigen, forming an antigen-antibody-colloidal gold complex. The complex then migrates through the nitrocellulose strip by capillary action. When the complex meets the line of immobilized antibody (Test line) "T", the complex is trapped forming an antibody-antigen-antibody colloidal gold complex. This forms a pink band indicating the sample is reactive for HBsAg. To serve as a procedural control, an additional line of anti-mouse antibody (Control line) "C", has been immobilized at a distance from the test line on the strip. If the test is performed correctly, this will result in the formation of a pink band upon contact with the conjugate.
- One step, rapid, visual and qualitative immunoassay for the detection of HBsAg.
- Detects all HBsAg subtypes.
- No sample preparation required.
- Ready to Use.
- Test cards, individually foil pouched with a desiccant.
- Package insert.
- Sample dispensing plastic dropper with each test pouch.
